By Iain Day
The identification and quantitation of low level impurities is essential in the drug development work flow, however, it becomes challenging when the intensity of the impurity signals becomes comparable to the 13C satellites of the parent compound. Impurity signals may lie close to the parent signals, especially if there are only minor changes in constitution. While trivial application of broadband 13C decoupling may address this problem, it comes with additional complications such as undesired sample heating.
In this poster we utilize the application of the DISPEL approach to remove the 13C satellites arising from 1JCH, without introducing additional sample heating. A comparison between DISPEL and broadband decoupled pulse acquire experiments will be shown, along with accurate quantification with JASON using quinine and extra virgin olive oil samples.