Quantitative NMR is one of the most important tools for the quantification of chemical species in samples. It is most commonly run through the measurement of 1H detected single pulse experiments, but severe spectral overlap makes the quantification of analytes a challenging task. 13C detected NMR alleviates the spectral overlap problem, and with modern instruments achieving higher sensitivity, 13C qNMR becomes a suitable alternative to 1H qNMR. Several aspects such as the acquisition of data with low signal-to-noise ratio and the larger bandwidth required to excite the 13C spectrum lead to a number of considerations that must be taken into account when acquiring 13C qNMR. Here we describe some of the practical aspects to take into account to acquire accurate 13C qNMR data.
